This protocol was adapted from the Australian protocol (Mary Bomford, 2008) by The Horus Institute and Dr. Leandro A. Pereira, currently at the Parana Federal Technical Institute (ITFPR), between 2008 and 2009.
Initially, 62 species were assessed to generate enough reference to adjust the protocol. Some of the species were indigenous in Brazil, displaced from their native range due to different interests or animal trafficking, often lacking references from other parts of the world. Some species were classic invaders: wild boar, bullfrog, rats, cat, dog, European hare, goat, sparrow, red eared slider.
Risk levels are: (a) very low; (b) low; (c) moderate; (d) high; and (e) very high. Moderate risk indicates the need for further assessment.
The precision of the protocol was estimated at 85% for the initial analyses. There was not much information available for some indigenous species that are not widespread, which tends to generate moderate risk results or invalid assessments. The classic invaders, however, were correctly recognized as of high risk.